Dual Labelled Probes
Dual Labelled Probes
We offer a broad spectrum of Probes for Real-Time PCR (TaqMan, Molecular Beacons, etc.) available in our premium grade DNA Reverse Phase HPLC.
Specification for DNA Reverse Phase HPLC (RP-HPLC) Dual Labelled Probes:
- Deprotected & purified
- Recommended for methods like PCR, sequencing and hybridization
- Delivered as solid
- Modification allowed
- Certificate of Analysis (CoA) follows
- 100% Quality Controlled (QC) by MassSpec
- To be kept frozen at -18 C
- Average in-house turnover time: 3-5 working days
- Scale & yield
Product | Synthesis scale (µmol) | Length | OD | Est. no. of PCR reactions |
---|---|---|---|---|
DNA Reverse Phase HPLC | 0.01 | n/a | n/a | n/a |
DNA Reverse Phase HPLC | 0.04 | 8-80 | 2 | 200 |
DNA Reverse Phase HPLC | 0.20 | 8-80 | 4 | 400 |
DNA Reverse Phase HPLC extra long | 0.20 | 81-120 | 2 | 200 |
DNA Reverse Phase HPLC | 1.00 | 8-80 | 20 | 20.000 |
How Dual Labeled Probes Work
A Dual-Labeled Probe is a single-stranded oligonucleotide labeled with two different dyes. A reporter dye is located at the 5’-end and a quencher molecule located at the 3′-end.
The quencher molecule inhibits the natural fluorescence emission of the reporter dye. The primer is elongated by the polymerase and the probe binds to the specific DNA template, hydrolysis releases the reporter dye from the probe/target hybrid, causing an increase of fluorescence. The measured fluorescence signal is directly proportional to the amount of target DNA.

How to Order
Please contact our customer service for any questions when ordering Dual Labelled Probes
You can also contact us directly via email or phone for personalized support.
Order Online
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Order by E-mail
Please send an e-mail to oligo@tagc.com and use our template by downloading it below:
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- Name
- Address
- Phone number
- Name of your institution
- Payment information
- The scale and sequence of the oligoes (one oligo per line)