PCR & qPCR Kit Specifications

PCR & qPCR Kit Specifications

TAG Copenhagen is the Danish representative for Solis BioDyne – a leading life science reagent supplier in Europe. The main focus of Solis Biodyne is the development of PCR & qPCR Reagents/Kits. Solis Biodyne offers the following PCR & qPCR products:

  • qPCR Mixes
  • PCR Mixes
  • Taq DNA Polymerases
  • Next Generation Sequencing Kits
  • Other PCR Reagents
  • Other Enzymes

A description of the PCR & qPCR Kits of Solis Biodyne is found below:

qPCR

Optimized ready-to-use solutions for real-time quantitative PCR assays with all the components necessary to perform qPCR.

  • Has mixes for both – probe based approach and intercalating dye approach (based on EvaGreen dye). Please find suitable mix for your qPCR platform from the table or product list below.
qPCR platformProbe basedIntercalating dye based
Applied Biosystems: 7900HT, 7500, 7700, 7000, StepOne™ & StepOnePlus™
Stratagene: Mx3000P™ & Mx3005P™
5x HOT FIREPol® Probe qPCR Mix Plus (ROX)5x HOT FIREPol® EvaGreen® qPCR Mix Plus (ROX)
Bio-Rad: CFX96™ & CFX384™, iQ™5 & MyiQ™,Chromo4™, Opticon® 2 & MiniOpticon®
Qiagen: Rotor-Gene® Q, Rotor-Gene® 6000
Eppendorf: Mastercycler®: ep realplex2 & ep
realplex4
Illumina: The Eco™
Roche: LightCycler® 480
5x HOT FIREPol® Probe qPCR Mix Plus (no ROX)5x HOT FIREPol® EvaGreen® qPCR Mix Plus (no ROX)
Roche: LightCycler® 1.x, LightCycler® 2.05x HOT FIREPol® Probe qPCR Mix Plus (Capillary)5x HOT FIREPol® EvaGreen® qPCR Mix Plus (Capillary)
Mix compatibility table

FIREPol is a trademark of Solis BioDyne. Applied Biosystems and ROX are trademarks of the Applera Corporation. Stratagene, MX3005, MX3005P are trademarks of Stratagene Corporation. Mastercycler is a trademark of Eppendorf AG. Rotor-Gene is a registered trademark of Corbett Research (Qiagen). CFX96, CFX384, iQ, MyiQ, Chromo4, Opticon 2, MiniOpticon are trademarks of Bio-Rad Laboratories. LightCycler is a registered trademark of Roche Applied Sciences. The Eco is registered trademark of Illumina Inc. EvaGreen is a registered trademark of BIOTIUM, INC.

ProductData sheetMSDS
HOT FIREPol® DNA PolymeraseData SheetMSDS
5x HOT FIREPol® Blend Master Mix Ready to Load with BSA and 7.5 mM MgCl2Data SheetMSDS
5x HOT FIREPol® Blend Master Mix Ready to Load with BSA and 10 mM MgCl2Data SheetMSDS
5x HOT FIREPol® Blend Master Mix Ready to Load with BSA and 12.5 mM MgCl2Data SheetMSDS
5x HOT FIREPol® Blend Master Mix Ready to Load with BSA and 15 mM MgCl2Data SheetMSDS

Hot Start PCR

  • Hot Start PCR prevents misextension from non-specifically annealed primers and primer-dimers.
  • HOT FIREPol®  DNA Polymerase is a chemically modified FIREPol®  DNA Polymerase. At ambient temperature it is inactive having no polymerization activity. HOT FIREPol® DNA Polymerase is activated by a 12-15 min incubation step at 95º C.
  • Hot-start master mixes are also available in ready-to-load format with variety of MgCl2 concentrations.
  • 5x HOT FIREPol® Blend Master Mix is a highly user-friendly and versatile tool for your Hot Start PCR. The mix is based on a blend of our HOT FIREPol®polymerase and a proofreading polymerase, providing a low error rate and high sensitivity. In addition, the mix also allows amplification of longer fragments (up to 5 kb). Here some great advantages of this ready-to-load mix: decreased set-up time
    • reduced risk of contamination
    • less pipetting mistakes
    • consistent reaction to reaction performance
    • contains all reagents required for PCR
  • Like all products of Solis BioDyne, the 5x HOT FIREPol® Blend Master Mix is stable at ambient temperature for up to one month. This makes handling and set-up even more convenient, since no ice has to be used.
ProductData sheetMSDS
HOT FIREPol® DNA PolymeraseData SheetMSDS
5x HOT FIREPol® Blend Master Mix Ready to Load with BSA and 7.5 mM MgCl2Data SheetMSDS
5x HOT FIREPol® Blend Master Mix Ready to Load with BSA and 10 mM MgCl2Data SheetMSDS
5x HOT FIREPol® Blend Master Mix Ready to Load with BSA and 12.5 mM MgCl2Data SheetMSDS
5x HOT FIREPol® Blend Master Mix Ready to Load with BSA and 15 mM MgCl2Data SheetMSDS

PCR

  • The polymerase chain reaction (PCR) is a technique to amplify a short sequence of DNA even in samples containing only minute quantities of DNA. PCR is used to amplify selected sections of DNA or RNA across several orders of magnitude.
  • FIREPol® is highly processive, thermostable and robust Taq polymerase with amplification range up to 3 kb. Due to Solis Tag technology, FIREPol® has an enhanced stability at ambient temperature with no activity loss up to 1 month.
  • We supply FIREPol® Polymerase as enzyme kit with a selection of buffers and an enhancer for personal optimization.
  • Master Mixes with or without ready-to-load feature are also available.
ProductData SheetMSDS
FIREPol® DNA PolymeraseData SheetMSDS
5x FIREPol® Master Mix (7.5 mM MgCl2)Data SheetMSDS
5x FIREPol® Master Mix (12.5 mM MgCl2)Data SheetMSDS
5x FIREPol® Master Mix Ready to Load (7.5 mM MgCl2)Data SheetMSDS
5x FIREPol® Master Mix Ready to Load (12.5 mM MgCl2)Data SheetMSDS

High Resolution Melt Analysis (HRM)

  • High Resolution Melting (HRM) is a hugely powerful, close-tube, post-qPCR genotyping method for the detection of mutations, polymorphisms and epigenetic differences in double stranded DNA samples.
  • It goes beyond the power of classical melting curve analysis (MCA) by allowing to study the thermal denaturation of a double-stranded DNA in much more detail. Samples can be discriminated according to their sequence, length, GC content or strand complementarity. Even single base changes such as SNPs (single nucleotide polymorphisms) can be readily identified.
  • HOT FIREPol® EvaGreen® HRM Mix is an optimized ready-to-use solution for High Resolution Melt (HRM) Analysis, incorporating EvaGreen® dye.
  • It includes:  HOT FIREPol® DNA Polymerase, ultrapure dNTPs, MgCl2 and ROX dye according to system requirements.
ProductData SheetMSDS
5x HOT FIREPol® EvaGreen® HRM Mix (no ROX)Data SheetMSDS
5x HOT FIREPol® EvaGreen® HRM Mix (ROX)Data SheetMSDS

High Resolution Melt Analysis (HRM)

  • High Resolution Melting (HRM) is a hugely powerful, close-tube, post-qPCR genotyping method for the detection of mutations, polymorphisms and epigenetic differences in double stranded DNA samples.
  • It goes beyond the power of classical melting curve analysis (MCA) by allowing to study the thermal denaturation of a double-stranded DNA in much more detail. Samples can be discriminated according to their sequence, length, GC content or strand complementarity. Even single base changes such as SNPs (single nucleotide polymorphisms) can be readily identified.
  • HOT FIREPol® EvaGreen® HRM Mix is an optimized ready-to-use solution for High Resolution Melt (HRM) Analysis, incorporating EvaGreen® dye.
  • It includes:  HOT FIREPol® DNA Polymerase, ultrapure dNTPs, MgCl2 and ROX dye according to system requirements.
ProductData SheetMSDS
5x HOT FIREPol® EvaGreen® HRM Mix (no ROX)Data SheetMSDS
5x HOT FIREPol® EvaGreen® HRM Mix (ROX)Data SheetMSDS

PCR & qPCR Kit Specifications & Price

  • The price for PCR & qPCR is available online – Click here

How to order

Orders are accepted by e-mail.

  • Name
  • Address
  • Phone number
  • Name of institution
  • Payment information for the order
  • Name of the PCR & qPCR Kits

Delivery & Handling

Delivery time

Delivery including in-house turnover time of PCR & qPCR Kits is 3-5 working days.

Countries

We offer delivery of PCR & qPCR Kits in Denmark, solely.

Shipping costs

Free delivery on orders of min. 300 DKK. Orders below, we charge 30 DKK within Denmark

Storage & handling

To be kept at room temperature for 30 days.