Phosphothioate Oligonucleotide


PTO (Phosphothioate Oligonucleotide) contain one sulfor atom in place of an oxygen atom in the internucleotide linkage of DNA or RNA. Phosphothioate oligonucleotide can be ordered as

  • Full PTO (whole oligo contains phosphothioate linkages)
  • “Thiocap” PTO (only a few phosphothioate linkages at the 3′ or 5′-end)
  • Chimera (mixed RNA/DNA with stretches of phosphothioate)

The more phosphothioate linkages present in the oligonucleotide, the higher the stability. Phosphothioate oligonucleotides are useful as antisense molecules inhibiting gene expression because of their resistance to nuclease degradation.

Phosphothioate oligonucleotides are manufactured to the specifications of our customers:


  • Unmodified PTO
  • Modified PTO
  • Plate PTO

Purification method:

  • Desalted
  • Reverse Phase Column


  • 40 nmol
  • 200 nmol
  • 1.000 nmol

Delivery method:

  • Solid
  • In 200 µL water
  • Standard Conc. (you add 100 µL water/buffer)
  • Conc. adjustment (multiple aliquoting and mixing)

In-house turnover time:

  • DNA PTO (3-5 days)
  • RNA PTO and Chimeric PTO (2-3 weeks)

Price list for PTO:

  • The price for PTO modification is available online – click here

How to order:

Please contact our customer service for a quotation and when ordering Phosphothioate oligonucleotide.