Dual Labelled Probes

Dual Labelled Probes

We offer a broad spectrum of Probes for Real-Time PCR (TaqMan, Molecular Beacons, etc.) available in our premium grade DNA Reverse Phase HPLC.

Specification for DNA Reverse Phase HPLC (RP-HPLC) Dual Labelled Probes:

  • Deprotected & purified
  • Recommended for methods like PCR, sequencing and hybridization
  • Delivered as solid
  • Modification allowed
  • Certificate of Analysis (CoA) follows
  • 100% Quality Controlled (QC) by MassSpec
  • To be kept frozen at -18 C
  • Average in-house turnover time: 3-5 working days
  • Scale & yield
ProductSynthesis scale (µmol)LengthODEst. no. of PCR reactions
DNA Reverse Phase HPLC0.01n/an/an/a
DNA Reverse Phase HPLC0.048-802200
DNA Reverse Phase HPLC0.208-804400
DNA Reverse Phase HPLC extra long0.2081-1202200
DNA Reverse Phase HPLC1.008-802020.000

List of available Dual Labelled Probes:

How Dual Labeled Probes Work

A Dual-Labeled Probe is a single-stranded oligonucleotide labeled with two different dyes. A reporter dye is located at the 5’-end and a quencher molecule located at the 3′-end. The quencher molecule inhibits the natural fluorescence emission of the reporter dye. The primer is elongated by the polymerase and the probe binds to the specific DNA template, hydrolysis releases the reporter dye from the probe/target hybrid, causing an increase of fluorescence. The measured fluorescence signal is directly proportional to the amount of target DNA.

Please inquire should you have any other requirements.

Price list for Dual Labelled Probes:

Please inquire should you have any other Oligo Modification requirements.

Order Online

Order by E-mail

Please send an e-mail to oligo@tagc.com and use our Excel template. Download HERE

If you do not wish to use our template, please add the following to your e-mail:
  • Name
  • Address
  • Phone number
  • Name of your institution
  • Payment information for the order
  • The scale and sequence of the oligoes (one oligo per line)